Soldati, C;
Lopez-Fabuel, I;
Wanderlingh, LG;
Garcia-Macia, M;
Monfregola, J;
Esposito, A;
Napolitano, G;
... Medina, DL; + view all
(2021)
Repurposing of tamoxifen ameliorates CLN3 and CLN7 disease phenotype.
EMBO Molecular Medicine
10.15252/emmm.202013742.
(In press).
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Abstract
Batten diseases (BDs) are a group of lysosomal storage disorders characterized by seizure, visual loss, and cognitive and motor deterioration. We discovered increased levels of globotriaosylceramide (Gb3) in cellular and murine models of CLN3 and CLN7 diseases and used fluorescent-conjugated bacterial toxins to label Gb3 to develop a cell-based high content imaging (HCI) screening assay for the repurposing of FDA-approved compounds able to reduce this accumulation within BD cells. We found that tamoxifen reduced the lysosomal accumulation of Gb3 in CLN3 and CLN7 cell models, including neuronal progenitor cells (NPCs) from CLN7 patient-derived induced pluripotent stem cells (iPSC). Here, tamoxifen exerts its action through a mechanism that involves activation of the transcription factor EB (TFEB), a master gene of lysosomal function and autophagy. In vivo administration of tamoxifen to the CLN7Δex2 mouse model reduced the accumulation of Gb3 and SCMAS, decreased neuroinflammation, and improved motor coordination. These data strongly suggest that tamoxifen may be a suitable drug to treat some types of Batten disease.
| Type: | Article |
|---|---|
| Title: | Repurposing of tamoxifen ameliorates CLN3 and CLN7 disease phenotype |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.15252/emmm.202013742 |
| Publisher version: | https://doi.org/10.15252/emmm.202013742 |
| Language: | English |
| Additional information: | © 2021 The Authors. Published under the terms of the CC BY 4.0 license This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Genetics and Genomic Medicine Dept |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10133638 |
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