Raffa, P;
Easler, M;
Cecchinato, F;
Auletta, B;
Scattolini, V;
Perin, S;
Gerli, MFM;
... Urciuolo, A; + view all
(2021)
Decellularized skeletal muscles support the generation of in vitro neuromuscular tissue models.
Applied Sciences
, 11
(20)
, Article 9485. 10.3390/app11209485.
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Abstract
Decellularized skeletal muscle (dSkM) constructs have received much attention in recent years due to the versatility of their applications in vitro. In search of adequate in vitro models of the skeletal muscle tissue, the dSkM offers great advantages in terms of the preservation of native-tissue complexity, including three-dimensional organization, the presence of residual signaling molecules within the construct, and their myogenic and neurotrophic abilities. Here, we attempted to develop a 3D model of neuromuscular tissue. To do so, we repopulated rat dSkM with human primary myogenic cells along with murine fibroblasts and we coupled them with organotypic rat spinal cord samples. Such culture conditions not only maintained multiple cell type viability in a long-term experimental setup, but also resulted in functionally active construct capable of contraction. In addition, we have developed a customized culture system which enabled easy access, imaging, and analysis of in vitro engineered co-cultures. This work demonstrates the ability of dSkM to support the development of a contractile 3D in vitro model of neuromuscular tissue fit for long-term experimental evaluations.
| Type: | Article |
|---|---|
| Title: | Decellularized skeletal muscles support the generation of in vitro neuromuscular tissue models |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3390/app11209485 |
| Publisher version: | https://doi.org/10.3390/app11209485 |
| Language: | English |
| Additional information: | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited |
| Keywords: | decellularized muscle; co-culture; neuromuscular model; 3D culture; skeletal muscle; spinal cord culture |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10137083 |
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