Maas, RGC;
Lee, S;
Harakalova, M;
Snijders Blok, CJB;
Goodyer, WR;
Hjortnaes, J;
Doevendans, PAFM;
... Buikema, JW; + view all
(2021)
Massive expansion and cryopreservation of functional human induced pluripotent stem cell-derived cardiomyocyte.
STAR Protocols
, 2
(1)
, Article 100334. 10.1016/j.xpro.2021.100334.
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Abstract
Since the discovery of human induced pluripotent stem cells (hiPSCs), numerous strategies have been established to efficiently derive cardiomyocytes from hiPSCs (hiPSC-CMs). Here, we describe a cost-effective strategy for the subsequent massive expansion (>250-fold) of high-purity hiPSC-CMs relying on two aspects: removal of cell-cell contacts and small-molecule inhibition with CHIR99021. The protocol maintains CM functionality, allows cryopreservation, and the cells can be used in downstream assays such as disease modeling, drug and toxicity screening, and cell therapy. For complete details on the use and execution of this protocol, please refer to Buikema (2020).
| Type: | Article |
|---|---|
| Title: | Massive expansion and cryopreservation of functional human induced pluripotent stem cell-derived cardiomyocyte |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.xpro.2021.100334 |
| Publisher version: | https://doi.org/10.1016/j.xpro.2021.100334 |
| Language: | English |
| Additional information: | © 2021 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| Keywords: | Cell culture, Cell differentiation, Stem cells |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Health Informatics UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10142705 |
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