Tognato, Riccardo;
(2022)
Theoretical investigation of erythrocytes optical trapping in ray optics approximation.
Doctoral thesis (Ph.D), UCL (University College London).
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Abstract
The thesis presents a theoretical investigation of the optical trapping of erythrocytes in the ray optics approximation. The thesis is divided in two parts: Part I provides an introduction on the general background on erythrocytes and the physics underlaying the work presented in the thesis; Part II presents the results obtained during my studies. In the first chapter of Part II, I introduce the ray-tracing scheme useful to perform the geometrical optics calculations for a healthy red blood cell that will be used extensively in the thesis. Therefore, I present a methodology for the identification of the equilibrium configuration of a red blood cell (RBC) for the simple case of a single-beam optical tweezers. Then, I proceed to investigate the equilibrium configuration of a RBC optically trapped with a double-, triple- and four-beam optical tweezers comparing my results with experiments. In the second chapter of Part II, I introduce a numerical scheme useful to simulate the Brownian dynamics of a non-spherical particle in a force-field (i.e. an optically trapped particle). This scheme is then applied to investigate the possibility to control the position and orientation of a healthy RBC with a reconfigurable triple-beam optical tweezers. In the third chapter of Part II, I investigate the possibility of optically confine and deliberately rotate a healthy RBC with a light-sheet optical tweezers (i.e. beam focused over a line instead of a point). In the fourth chapter of Part II, I present the research carried out in collaboration with Nano-Soft Lab at CNR in Messina, Italy. Here we couple the geometrical optics calculation with machine learning to improve the accuracy and the speed of geometrical optics calculations. Lastly, in the fifth chapter of Part II, I extend the work presented in the previous chapters to a pathological RBC conformation (i.e. sickle cell).
| Type: | Thesis (Doctoral) |
|---|---|
| Qualification: | Ph.D |
| Title: | Theoretical investigation of erythrocytes optical trapping in ray optics approximation |
| Open access status: | An open access version is available from UCL Discovery |
| Language: | English |
| Additional information: | Copyright © The Author 2022. Original content in this thesis is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) Licence (https://creativecommons.org/licenses/by-nc/4.0/). Any third-party copyright material present remains the property of its respective owner(s) and is licensed under its existing terms. Access may initially be restricted at the author’s request. |
| UCL classification: | UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy UCL > Provost and Vice Provost Offices > UCL BEAMS UCL |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10148700 |
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