Hammond, Katharine;
Moffat, Jonathan;
Mulcahy, Chris;
Hoogenboom, Bart W;
Ryadnov, Maxim G;
(2022)
In situ nanoscale imaging reveals self-concentrating nanomolar antimicrobial pores.
Nanoscale
, 14
(24)
pp. 8586-8593.
10.1039/d2nr00434h.
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Abstract
Host defence peptides are critical factors of immune systems in all life forms. Considered for therapeutic development in the post-antibiotic era, these molecules rupture microbial membranes at micromolar concentrations. Here we report a self-concentrating mechanism of membrane disruption, which occurs at therapeutically more relevant nanomolar concentrations. Induced by a four-helix bacteriocin the mechanism manifests in a multi-modal disruption pattern. Using in situ atomic force microscopy we show that the pattern and its kinetic profiles remain the same in a range of nano-to-micromolar concentrations. We reveal that the bacteriocin creates its own boundaries in phospholipid bilayers in which it self-concentrates to promote transmembrane poration. The findings offer an exploitable insight into nanomolar antimicrobial mechanisms.
| Type: | Article |
|---|---|
| Title: | In situ nanoscale imaging reveals self-concentrating nanomolar antimicrobial pores |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1039/d2nr00434h |
| Publisher version: | https://doi.org/10.1039/d2nr00434h |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| UCL classification: | UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy UCL > Provost and Vice Provost Offices > UCL BEAMS UCL |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10149721 |
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