Chung, Chih-Yao;
Duchen, Michael R;
(2022)
A Plate Reader-Based Measurement of the Cellular ROS Production Using Dihydroethidium and MitoSOX.
Methods in Molecular Biology
, 2497
pp. 333-337.
10.1007/978-1-0716-2309-1_24.
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Abstract
Intracellular reactive oxygen species (ROS) act as an important signaling transductor in cells, regulating almost every aspect of cell biology. Measurements of ROS production thus, offer links between oxidative stress and cell pathophysiology. Here, we describe a simple screening assay in intact adherent cells by fluorescence microplate readers, using dihydroethidium (DHE) and MitoSOX to measure cytosolic superoxide and mitochondrial superoxide production, respectively. This assay enables a quick and reliable assessment of ROS generation in a well-controlled environment.
| Type: | Article |
|---|---|
| Title: | A Plate Reader-Based Measurement of the Cellular ROS Production Using Dihydroethidium and MitoSOX |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-0716-2309-1_24 |
| Publisher version: | https://doi.org/10.1007/978-1-0716-2309-1_24 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Dihydroethidium, MitoSOX, Mitochondria, Plate reader, Reactive oxygen species, Ethidium, Oxidative Stress, Phenanthridines, Reactive Oxygen Species, Superoxides |
| UCL classification: | UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10152151 |
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