Chi, Haoyu;
Bhosale, Gauri;
Duchen, Michael R;
(2022)
Assessing the Redox Status of Mitochondria Through the NADH/FAD²⁺ Ratio in Intact Cells.
Methods in Molecular Biology
, 2497
pp. 313-318.
10.1007/978-1-0716-2309-1_21.
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Abstract
This section aims to describe the measurement of NADH and FAD2+ levels in intact cells using fluorescence microscopy. Both NADH and FADH2 are major electron donors for the electron transport chain through shifting of their redox status. Furthermore, within their redox couples, only NADH and FAD2+ are fluorescent. Therefore, calibration of the NADH and FAD2+ fluorescence signal is a crucial factor in accurately assessing mitochondrial function and redox status.
| Type: | Article |
|---|---|
| Title: | Assessing the Redox Status of Mitochondria Through the NADH/FAD²⁺ Ratio in Intact Cells |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-0716-2309-1_21 |
| Publisher version: | https://doi.org/10.1007/978-1-0716-2309-1_21 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Autofluorescence, ETC, FAD2+, NADH, Redox status, Flavin-Adenine Dinucleotide, Microscopy, Fluorescence, Mitochondria, NAD, Oxidation-Reduction |
| UCL classification: | UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10152154 |
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