Valdebenito, Gabriel Esteban;
Duchen, Michael R;
(2022)
Monitoring Mitochondrial Membrane Potential in Live Cells Using Time-Lapse Fluorescence Imaging.
Methods in Molecular Biology
, 2497
pp. 319-324.
10.1007/978-1-0716-2309-1_22.
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Abstract
The mitochondrial membrane potential (ΔΨm) generated by proton pumps (Complexes I, III, and IV) is an essential component in the process of energy generation during oxidative phosphorylation. Tetramethylrhodamine, methyl ester, perchlorate (TMRM) is one of the most commonly used fluorescent reporters of ΔΨm. TMRM is routinely employed in a steady state for the measurement of membrane potential. However, it can also be utilized with time-lapse fluorescence imaging to effectively monitor the changes in membrane potential in response to a given stimulus by analyzing the change in distribution of the dye with time.
| Type: | Article |
|---|---|
| Title: | Monitoring Mitochondrial Membrane Potential in Live Cells Using Time-Lapse Fluorescence Imaging |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-0716-2309-1_22 |
| Publisher version: | https://doi.org/10.1007/978-1-0716-2309-1_22 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Fluorescence microscopy, Mitochondria membrane potential, Primary skin fibroblasts, TMRM, Uncoupler, Cells, Cultured, Membrane Potential, Mitochondrial, Mitochondria, Optical Imaging, Time-Lapse Imaging |
| UCL classification: | UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10152156 |
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