Suthar, Jugal;
Taub, Marissa;
Carney, Randy P;
Williams, Gareth R;
Guldin, Stefan;
(2022)
Recent developments in biosensing methods for extracellular vesicle protein characterization.
Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology
10.1002/wnan.1839.
(In press).
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Abstract
Research into extracellular vesicles (EVs) has grown significantly over the last few decades with EVs being widely regarded as a source of biomarkers for human health and disease with massive clinical potential. Secreted by every cell type in the body, EVs report on the internal cellular conditions across all tissue types. Their presence in readily accessible biofluids makes the potential of EV biosensing highly attractive as a noninvasive diagnostic platform via liquid biopsies. However, their small size (50-250 nm), inherent heterogeneity, and the complexity of the native biofluids introduce challenges for effective characterization, thus, limiting their clinical utility. This has led to a surge in the development of various novel EV biosensing techniques, with capabilities beyond those of conventional methods that have been directly transferred from cell biology. In this review, key detection principles used for EV biosensing are summarized, with a focus on some of the most recent and fundamental developments in the field over the last 5 years. This article is categorized under: Diagnostic Tools > Biosensing Diagnostic Tools > In Vitro Nanoparticle-Based Sensing.
| Type: | Article |
|---|---|
| Title: | Recent developments in biosensing methods for extracellular vesicle protein characterization |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/wnan.1839 |
| Publisher version: | https://doi.org/10.1002/wnan.1839 |
| Language: | English |
| Additional information: | © 2022 The Authors. WIREs Nanomedicine and Nanobiotechnology published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | absorbance, acoustic resonators, biosensing, electrochemical, electrochemical quartz crystal microbalance with dissipation, exosomes, extracellular vesicles, fluorescence, interferometry, plasmon resonance, surface enhanced Raman spectroscopy |
| UCL classification: | UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Chemical Engineering UCL > Provost and Vice Provost Offices > UCL BEAMS |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10154798 |
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