Li, Yixuan;
Luo, Liuxiong;
Nie, Mengyan;
Davenport, Andrew;
Li, Ying;
Li, Bing;
Choy, Kwang-Leong;
(2022)
A graphene nanoplatelet-polydopamine molecularly imprinted biosensor for Ultratrace creatinine detection.
Biosensors and Bioelectronics
, 216
, Article 114638. 10.1016/j.bios.2022.114638.
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Abstract
Accurate and reliable analysis of creatinine is clinically important for the early detection and monitoring of patients with kidney disease. We report a novel graphene nanoplatelet (GNP)/polydopamine (PDA)-molecularly imprinted polymer (MIP) biosensor for the ultra-trace detection of creatinine in a range of body fluids. Dopamine hydrochloride (DA) monomers were polymerized using a simple one-pot method to form a thin PDA-MIP layer on the surface of GNP with high density of creatinine recognition sites. This novel surface-MIP strategy resulted in a record low limit-of-detection (LOD) of 2 × 10^{−2} pg/ml with a wide dynamic detection range between 1 × 10^{−1}-1 × 10^{9} pg/ml. The practical application of this GNP/PDA-MIP biosensor has been tested by measuring creatinine in human serum, urine, and peritoneal dialysis (PD) fluids. The average recovery rate was 93.7–109.2% with relative standard deviation (RSD) below 4.1% compared to measurements made using standard clinical laboratory methods. Our GNP/PDA-MIP biosensor holds high promise for further development as a rapid, accurate, point-of-care diagnostic platform for detecting and monitoring patients with kidney disease.
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