Rusakov, Dmitri A;
(2022)
Avoiding interpretational pitfalls in fluorescence imaging of the brain.
Nature Reviews Neuroscience
, 23
pp. 705-706.
10.1038/s41583-022-00643-z.
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Abstract
Fluorescent sensors of molecular activity have revolutionized our knowledge of the brain. However, their signals report a reaction between the target and the sensor molecules rather than the activity of interest per se. Thus, understanding the location, sensitivity and imaging environment of a sensor should help to avoid misinterpretation of its readout.
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