Webby, Melissa N;
Oluwole, Abraham O;
Pedebos, Conrado;
Inns, Patrick G;
Olerinyova, Anna;
Prakaash, Dheeraj;
Housden, Nicholas G;
... Kleanthous, Colin; + view all
(2022)
Lipids mediate supramolecular outer membrane protein assembly in bacteria.
Science Advances
, 8
(44)
, Article eadc9566. 10.1126/sciadv.adc9566.
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Abstract
β Barrel outer membrane proteins (OMPs) cluster into supramolecular assemblies that give function to the outer membrane (OM) of Gram-negative bacteria. How such assemblies form is unknown. Here, through photoactivatable cross-linking into the Escherichia coli OM, coupled with simulations, and biochemical and biophysical analysis, we uncover the basis for OMP clustering in vivo. OMPs are typically surrounded by an annular shell of asymmetric lipids that mediate higher-order complexes with neighboring OMPs. OMP assemblies center on the abundant porins OmpF and OmpC, against which low-abundance monomeric β barrels, such as TonB-dependent transporters, are packed. Our study reveals OMP-lipid-OMP complexes to be the basic unit of supramolecular OMP assembly that, by extending across the entire cell surface, couples the requisite multifunctionality of the OM to its stability and impermeability.
| Type: | Article |
|---|---|
| Title: | Lipids mediate supramolecular outer membrane protein assembly in bacteria |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1126/sciadv.adc9566 |
| Publisher version: | https://doi.org/10.1126/sciadv.adc9566 |
| Language: | English |
| Additional information: | © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S.Government Works. Distributed under a Creative Commons Attribution License 4.0 (https://creativecommons.org/licenses/by/4.0/). |
| Keywords: | Escherichia coli Proteins, Bacterial Outer Membrane Proteins, Escherichia coli, Cell Membrane, Lipids |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10158916 |
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