Piatkowska, Agnieszka M;
Adhikari, Kaustubh;
Moverley, Adam A;
Turmaine, Mark;
Glazier, James A;
Plachta, Nicolas;
Evans, Susan E;
(2022)
Sequential changes in cellular properties accompanying amniote somite formation.
Journal of Anatomy
10.1111/joa.13791.
(In press).
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Journal of Anatomy - 2022 - Piatkowska - Sequential changes in cellular properties accompanying amniote somite formation.pdf - Published Version Download (9MB) | Preview |
Abstract
Somites are transient structures derived from the pre-somitic mesoderm (PSM), involving mesenchyme-to-epithelial transition (MET) where the cells change their shape and polarize. Using Scanning electron microscopy (SEM), immunocytochemistry and confocal microscopy, we study the progression of these events along the tail-to-head axis of the embryo, which mirrors the progression of somitogenesis (younger cells located more caudally). SEM revealed that PSM epithelialization is a gradual process, which begins much earlier than previously thought, starting with the dorsalmost cells, then the medial ones, and then, simultaneously, the ventral and lateral cells, before a somite fully separates from the PSM. The core (internal) cells of the PSM and somites never epithelialize, which suggests that the core cells could be 'trapped' within the somitocoele after cells at the surfaces of the PSM undergo MET. Three-dimensional imaging of the distribution of the cell polarity markers PKCζ, PAR3, ZO1, the Golgi marker GM130 and the apical marker N-cadherin reveal that the pattern of polarization is distinctive for each marker and for each surface of the PSM, but the order of these events is not the same as the progression of cell elongation. These observations challenge some assumptions underlying existing models of somite formation.
| Type: | Article |
|---|---|
| Title: | Sequential changes in cellular properties accompanying amniote somite formation |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1111/joa.13791 |
| Publisher version: | https://doi.org/10.1111/joa.13791 |
| Language: | English |
| Additional information: | © 2022 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | cell adhesion, cell polarity, epithelial-mesenchymal transition, mesenchymal-epithelial transition, segmentation, vertebral column |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10160600 |
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