He, Peng;
Lim, Kyungtae;
Sun, Dawei;
Pett, Jan Patrick;
Jeng, Quitz;
Polanski, Krzysztof;
Dong, Ziqi;
... Rawlins, Emma L; + view all
(2022)
A human fetal lung cell atlas uncovers proximal-distal gradients of differentiation and key regulators of epithelial fates.
Cell
, 185
(25)
4841-4860.e25.
10.1016/j.cell.2022.11.005.
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Abstract
We present a multiomic cell atlas of human lung development that combines single-cell RNA and ATAC sequencing, high-throughput spatial transcriptomics, and single-cell imaging. Coupling single-cell methods with spatial analysis has allowed a comprehensive cellular survey of the epithelial, mesenchymal, endothelial, and erythrocyte/leukocyte compartments from 5-22 post-conception weeks. We identify previously uncharacterized cell states in all compartments. These include developmental-specific secretory progenitors and a subtype of neuroendocrine cell related to human small cell lung cancer. Our datasets are available through our web interface (https://lungcellatlas.org). To illustrate its general utility, we use our cell atlas to generate predictions about cell-cell signaling and transcription factor hierarchies which we rigorously test using organoid models.
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