Memar, Nadin;
Sethi, Aditya;
Luehr, Sebastian;
Lambie, Eric J;
Conradt, Barbara;
(2022)
In vivo labeling of endogenous genomic loci in C. elegans using CRISPR/dCas9.
microPublication Biology
, 2022
10.17912/micropub.biology.000701.
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Abstract
Visualization of genomic loci with open chromatin state has been reported in mammalian tissue culture cells using a CRISPR/Cas9-based system that utilizes an EGFP-tagged endonuclease-deficient Cas9 protein (dCas9::EGFP) (Chen et al. 2013). Here, we adapted this approach for use in Caenorhabditis elegans . We generated a C. elegans strain that expresses the dCas9 protein fused to two nuclear-localized EGFP molecules (dCas9::NLS::2xEGFP::NLS) in an inducible manner. Using this strain, we report the visualization in live C. elegans embryos of two endogenous repetitive loci, rrn-4 and rrn-1 , from which 5S and 18S ribosomal RNAs are constitutively generated.
| Type: | Article |
|---|---|
| Title: | In vivo labeling of endogenous genomic loci in C. elegans using CRISPR/dCas9 |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.17912/micropub.biology.000701 |
| Publisher version: | https://doi.org/10.17912/micropub.biology.000701 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third-party material in this article are included in the Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10163397 |
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