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Experiments and simulations demonstrating the rapid ultrasonic rewarming of frozen tissue cryovials

Xu, R; Treeby, BE; Martin, E; (2023) Experiments and simulations demonstrating the rapid ultrasonic rewarming of frozen tissue cryovials. Journal of the Acoustical Society of America , 153 (1) pp. 517-528. 10.1121/10.0016886. Green open access

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Abstract

The development of methods to safely rewarm large cryopreserved biological samples remains a barrier to the widespread adoption of cryopreservation. Here, experiments and simulations were performed to demonstrate that ultrasound can increase rewarming rates relative to thermal conduction alone. An ultrasonic rewarming setup based on a custom 444 kHz tubular piezoelectric transducer was designed, characterized, and tested with 2 ml cryovials filled with frozen ground beef. Rewarming rates were characterized in the -20 °C to 5 °C range. Thermal conduction-based rewarming was compared to thermal conduction plus ultrasonic rewarming, demonstrating a tenfold increase in rewarming rate when ultrasound was applied. The maximum recorded rewarming rate with ultrasound was 57° C/min, approximately 2.5 times faster than with thermal conduction alone. Coupled acoustic and thermal simulations were developed and showed good agreement with the heating rates demonstrated experimentally and were also used to demonstrate spatial heating distributions with small (<3° C) temperature differentials throughout the sample when the sample was below 0° C. The experiments and simulations demonstrate the potential for ultrasonic cryovial rewarming with a possible application to large volume rewarming, as faster rewarming rates may improve the viability of cryopreserved tissues and reduce the time needed for cells to regain normal function.

Type: Article
Title: Experiments and simulations demonstrating the rapid ultrasonic rewarming of frozen tissue cryovials
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1121/10.0016886
Publisher version: https://doi.org/10.1121/10.0016886
Language: English
Additional information: © 2023 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). https://doi.org/10.1121/10.0016886
Keywords: Animals, Cattle, Rewarming, Ultrasonics, Cryopreservation, Temperature, Transducers
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng
URI: https://discovery-pp.ucl.ac.uk/id/eprint/10164581
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