Weiler, Simon;
Guggiana Nilo, Drago;
Bonhoeffer, Tobias;
Hübener, Mark;
Rose, Tobias;
Scheuss, Volker;
(2022)
Functional and structural features of L2/3 pyramidal cells continuously covary with pial depth in mouse visual cortex.
Cerebral Cortex
, Article bhac303. 10.1093/cercor/bhac303.
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Weiler et al. 2022 Functional and structural features of L23 pyramidal cells continuously covary with pial depth in mouse visual cortex.pdf - Accepted Version Download (2MB) | Preview |
Abstract
Pyramidal cells of neocortical layer 2/3 (L2/3 PyrCs) integrate signals from numerous brain areas and project throughout the neocortex. These PyrCs show pial depth-dependent functional and structural specializations, indicating participation in different functional microcircuits. However, whether these depth-dependent differences result from separable PyrC subtypes or whether their features display a continuum correlated with pial depth is unknown. Here, we assessed the stimulus selectivity, electrophysiological properties, dendritic morphology, and excitatory and inhibitory connectivity across the depth of L2/3 in the binocular visual cortex of mice. We find that the apical, but not the basal dendritic tree structure, varies with pial depth, which is accompanied by variation in subthreshold electrophysiological properties. Lower L2/3 PyrCs receive increased input from L4, while upper L2/3 PyrCs receive a larger proportion of intralaminar input. In vivo calcium imaging revealed a systematic change in visual responsiveness, with deeper PyrCs showing more robust responses than superficial PyrCs. Furthermore, deeper PyrCs are more driven by contralateral than ipsilateral eye stimulation. Importantly, the property value transitions are gradual, and L2/3 PyrCs do not display discrete subtypes based on these parameters. Therefore, L2/3 PyrCs' multiple functional and structural properties systematically correlate with their depth, forming a continuum rather than discrete subtypes.
| Type: | Article |
|---|---|
| Title: | Functional and structural features of L2/3 pyramidal cells continuously covary with pial depth in mouse visual cortex |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1093/cercor/bhac303 |
| Publisher version: | https://doi.org/10.1093/cercor/bhac303 |
| Language: | English |
| Additional information: | © The Author(s) 2022. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | L2/3 pyramidal cells, continuum, cortical circuit mapping, in vivo 2-photon imaging, visual cortex |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > The Sainsbury Wellcome Centre |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10164910 |
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