Tedeschi, Alfonso Maria;
(2023)
Mesoangioblast promote regeneration of a stable and organised vasculature in a decellularised intestinal graft.
Doctoral thesis (Ph.D), UCL (University College London).
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Abstract
Whole organ tissue engineering has emerged as a viable solution to the lack of organs for transplant, but there is a significant bottleneck in whole organ tissue creation, namely the vasculature. The main cell types that compose the vasculature are endothelial cells and perivascular cells. Recent efforts used endothelial cells and supporting cells to engineer organs’ vasculature. Ott’s group published in 2015 a pioneer study introducing this concept and seeding both cell types through both arterial and venous routes in a rat decellularised lung. Nevertheless, still many works attempted organ revascularisation without taking in account the perivascular compound. Moreover, in many works, the presence of the engineered graft has been characterised only after few days in culture in vitro and in vivo and sustainability over time of the engineered endothelium is another point that need to be addressed In this context, this work aims to elucidate the mechanisms behind which endothelial cells (Human Umbilical Vein Endothelial Cells, HUVECs) and perivascular cells (Mesoangioblasts, MABs) cross-talk and repopulated the preserved vasculature of a decellularised whole organ. In this study I report the use of the perivascular compartment as pivotal for generating a vasculature with good barrier function and durability both in vitro and in vivo, with poor results obtained instead when only HUVECs were used. In this work I established a perfusable, functional, and long-lasting vasculature with cells organising themselves in a native manner by seeding MABs and HUVECs into the conserved vasculature of a decellularised rat gut.
| Type: | Thesis (Doctoral) |
|---|---|
| Qualification: | Ph.D |
| Title: | Mesoangioblast promote regeneration of a stable and organised vasculature in a decellularised intestinal graft |
| Open access status: | An open access version is available from UCL Discovery |
| Language: | English |
| Additional information: | Copyright © The Author 2022. Original content in this thesis is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) Licence (https://creativecommons.org/licenses/by-nc/4.0/). Any third-party copyright material present remains the property of its respective owner(s) and is licensed under its existing terms. Access may initially be restricted at the author’s request. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10165174 |
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