Liu, G;
Riemer, E;
Schneider, R;
Cabuzu, D;
Bonny, O;
Wagner, CA;
Qiu, D;
... Jessen, HJ; + view all
(2023)
The phytase RipBL1 enables the assignment of a specific inositol phosphate isomer as a structural component of human kidney stones.
RSC Chemical Biology
10.1039/d2cb00235c.
(In press).
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Abstract
Inositol phosphates (InsPs) are ubiquitous in all eukaryotes. However, since there are 63 possible different phosphate ester isomers, the analysis of InsPs is challenging. In particular, InsP1, InsP2, and InsP3 already amass 41 different isomers, of which some occur as enantiomers. Profiling of these “lower” inositol phosphates in mammalian tissues requires powerful analytical methods and reference compounds. Here, we report an analysis of InsP2 and InsP3 with capillary electrophoresis coupled to electrospray ionization mass spectrometry (CE-ESI-MS). Using this method, the bacterial effector RipBL1 was analyzed and found to degrade InsP6 to Ins(1,2,3)P3, an understudied InsP3 isomer. This new reference molecule then aided us in the assignment of the isomeric identity of an InsP3 while profiling human samples: in urine and kidney stones, we describe for the first time the presence of defined and abundant InsP3 isomers, namely Ins(1,2,3)P3, Ins(1,2,6)P3 and/or Ins(2,3,4)P3
| Type: | Article |
|---|---|
| Title: | The phytase RipBL1 enables the assignment of a specific inositol phosphate isomer as a structural component of human kidney stones |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1039/d2cb00235c |
| Publisher version: | https://doi.org/10.1039/d2cb00235c |
| Language: | English |
| Additional information: | This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10165821 |
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