Sant, Damini; Rojekar, Satish; Gera, Sakshi; Pallapati, Anusha R; Gimenez-Roig, Judit; Kuo, Tan-Chun; Padilla, Ashley; ... Zaidi, Mone; + view all Sant, Damini; Rojekar, Satish; Gera, Sakshi; Pallapati, Anusha R; Gimenez-Roig, Judit; Kuo, Tan-Chun; Padilla, Ashley; Korkmaz, Funda; Cullen, Liam; Chatterjee, Jiya; Shelly, Eleanor; Meseck, Marcia; Miyashita, Sari; Macdonald, Anne; Sultana, Farhath; Barak, Orly; Ryu, Vitaly; Kim, Se-Min; Robinson, Cemre; Rosen, Clifford J; Caminis, John; Lizneva, Daria; Haider, Shozeb; Yuen, Tony; Zaidi, Mone; - view fewer (2023) Optimizing a therapeutic humanized follicle-stimulating hormone-blocking antibody formulation by protein thermal shift assay. Annals of the New York Academy of Sciences , 1521 (1) pp. 67-78. 10.1111/nyas.14952.

Preview

Text Shozeb_Sant et al Ann NY Acad Sci 2023.pdf Download (245kB) | Preview

Abstract

Biopharmaceutical products are formulated using several Food and Drug Administration (FDA) approved excipients within the inactive ingredient limit to maintain their storage stability and shelf life. Here, we have screened and optimized different sets of excipient combinations to yield a thermally stable formulation for the humanized follicle-stimulating hormone (FSH)-blocking antibody, MS-Hu6. We used a protein thermal shift assay in which rising temperatures resulted in the maximal unfolding of the protein at the melting temperature (Tm ). To determine the buffer and pH for a stable solution, four different buffers with a pH range from 3 to 8 were screened. This resulted in maximal Tm s at pH 5.62 for Fab in phosphate buffer and at pH 6.85 for Fc in histidine buffer. Upon testing a range of salt concentrations, MS-Hu6 was found to be more stable at lower concentrations, likely due to reduced hydrophobic effects. Molecular dynamics simulations revealed a higher root-mean-square deviation with 1 mM than with 100 mM salt, indicating enhanced stability, as noted experimentally. Among the stabilizers tested, Tween 20 was found to yield the highest Tm and reversed the salt effect. Among several polyols/sugars, trehalose and sucrose were found to produce higher thermal stabilities. Finally, binding of recombinant human FSH to MS-Hu6 in a final formulation (20 mM phosphate buffer, 1 mM NaCl, 0.001% w/v Tween 20, and 260 mM trehalose) resulted in a thermal shift (increase in Tm ) for the Fab, but expectedly not in the Fc domain. Given that we used a low dose of MS-Hu6 (1 μM), the next challenge would be to determine whether 100-fold higher, industry-standard concentrations are equally stable.

Download activity - last month

Export as JSONCSVXML

Download activity - last 12 months

Export as XMLCSVJSON

Downloads by country - last 12 months

Export as CSVXMLJSON

Archive Staff Only

View Item