Chung, Gary Hong Chun;
Domart, Marie-Charlotte;
Peddie, Christopher;
Mantell, Judith;
Mclaverty, Kieran;
Arabiotorre, Angela;
Hodgson, Lorna;
... Larijani, Banafshé; + view all
(2018)
Acute depletion of diacylglycerol from the cis-Golgi affects localized nuclear envelope morphology during mitosis.
Journal of Lipid Research
, 59
(8)
pp. 1402-1413.
10.1194/jlr.M083899.
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Abstract
Dysregulation of nuclear envelope (NE) assembly results in various cancers; for example, renal and some lung carcinomas ensue due to NE malformation. The NE is a dynamic membrane compartment and its completion during mitosis is a highly regulated process, but the detailed mechanism still remains incompletely understood. Previous studies have found that isolated diacylglycerol (DAG)-containing vesicles are essential for completing the fusion of the NE in nonsomatic cells. We investigated the impact of DAG depletion from the cis-Golgi in mammalian cells on NE reassembly. Using advanced electron microscopy, we observed an enriched DAG population of vesicles at the vicinity of the NE gaps of telophase mammalian cells. We applied a mini singlet oxygen generator-C1-domain tag that localized DAG-enriched vesicles at the perinuclear region, which suggested the existence of NE fusogenic vesicles. We quantified the impact of Golgi-DAG depletion by measuring the in situ NE rim curvature of the reforming NE. The rim curvature in these cells was significantly reduced compared with controls, which indicated a localized defect in NE morphology. Our novel results demonstrate the significance of the role of DAG from the cis-Golgi for the regulation of NE assembly.
| Type: | Article |
|---|---|
| Title: | Acute depletion of diacylglycerol from the cis-Golgi affects localized nuclear envelope morphology during mitosis |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1194/jlr.M083899 |
| Publisher version: | http://doi.org/10.1194/jlr.M083899 |
| Language: | English |
| Additional information: | Final version open access under the terms of the Creative Commons CC-BY license (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | correlative light electron microscopy, membrane curvature, serial block face scanning electron microscopy, Diglycerides, Golgi Apparatus, HeLa Cells, Humans, Mitosis, Nuclear Envelope |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10166354 |
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