Luthuli, Bonisile;
Gounder, Kamini;
Deymier, Martin J;
Dong, Krista L;
Balazs, Alejandro B;
Mann, Jaclyn K;
Ndung'u, Thumbi;
(2023)
Generation and characterization of infectious molecular clones of transmitted/founder HIV-1 subtype C viruses.
Virology
, 583
pp. 14-26.
10.1016/j.virol.2023.04.001.
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Abstract
The genetic diversity of HIV impedes vaccine development. Identifying the viral properties of transmitted/founder (T/F) variants may provide a common vaccine target. To study the biological nature of T/F viruses, we constructed full-length clones from women detected during Fiebig stage I acute HIV-1 infection (AHI) from heterosexual male-to-female (MTF) transmission; and clones after one year of infection using In-Fusion-based cloning. Eighteen full-length T/F clones were generated from 9 women and six chronic infection clones were from 2 individuals. All clones but one were non-recombinant subtype C. Three of the 5 T/F clones and 3 chronic clones tested replicated efficiently in PBMCs and utilised CCR5 coreceptor for cell entry. Transmitted/founder and chronic infection clones displayed heterogenous in vitro replicative capacity and resistance to type I interferon. T/F viruses had shorter Env glycoproteins and fewer N-linked glycosylation sites in Env. Our findings suggest MTF transmission may select viruses with compact envelopes.
| Type: | Article |
|---|---|
| Title: | Generation and characterization of infectious molecular clones of transmitted/founder HIV-1 subtype C viruses |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.virol.2023.04.001 |
| Publisher version: | https://doi.org/10.1016/j.virol.2023.04.001 |
| Language: | English |
| Additional information: | © 2023 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | HIV-1, Subtype C, Transmitted/founder, Infectious molecular clones |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10168665 |
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