Savvidis, Savvas;
Gerli, Mattia F;
Citro, Antonio;
Massimi, Lorenzo;
Hagen, Charlotte K;
Endrizzi, Marco;
Atzeni, Alessia;
... Olivo, Alessandro; + view all
(2023)
Advanced x-ray imaging techniques in tissue engineering: a new construct assessment platform for enabling the regeneration of personalised organs.
In:
Proceedings of SPIE: Medical Imaging 2023: Biomedical Applications in Molecular, Structural, and Functional Imaging.
Society of Photo-Optical Instrumentation Engineers (SPIE)
Preview |
Text
Olivo_Advanced x-ray imaging techniques in tissue engineering_VoR.pdf Download (6MB) | Preview |
Abstract
Tissue engineering (TE) holds promise for generating lab-grown patient specific organs which can provide: (1) effective treatment for conditions that require volumetric tissue transplantation and (2) new platforms for drug testing. Even though volumetric structural information is essential for confirming successful organ maturation, TE protocol designs are currently informed through destructive and 2D construct assessment tools (e.g. histology). X-ray phase-contrast computed-tomography (PC-CT) can generate non-destructive, high resolution, 3D density maps of organ architecture. In this work, PC-CT is used as new imaging tool for guiding two TE protocols currently at the in-vitro testing stage. The first (1) involves cell-repopulation of an oesophageal scaffold, with the aim of using the regenerated construct for treating long-gap oesophageal atresia, whilst for the second (2) a lung-derived scaffold is populated with islets for regenerating a pancreas, with the “repurposed” lung offering a platform for diabetes drug testing. By combing 3D images and quantitative information, we were able to perform comprehensive construct evaluation. Specifically, we assessed volumetrically: (1) the cell-distribution within the regenerated oesophagi and (2) islet integration with the vascular tree of the lung-derived scaffold. This new information was proven to be essential for establishing corresponding TE protocols and enabled their progression to more advanced scale-up models. We are confident that PC-CT will provide the novel insights necessary to further progress TE protocols, with the next step being in-vivo testing. Crucially, the non-destructive nature of PC-CT will allow in-vivo assessments of TE constructs following their implantation into animal hosts, to investigate their successful integration.
| Type: | Proceedings paper |
|---|---|
| Title: | Advanced x-ray imaging techniques in tissue engineering: a new construct assessment platform for enabling the regeneration of personalised organs |
| Event: | Biomedical Applications in Molecular, Structural, and Functional Imaging |
| Dates: | 19 Feb 2023 - 24 Feb 2023 |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1117/12.2653075 |
| Publisher version: | https://doi.org/10.1117/12.2653075 |
| Language: | English |
| Additional information: | This version is the version of record. For information on re-use, please refer to the publisher's terms and conditions. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10169563 |
Archive Staff Only
 |
View Item |
