Hannabuss, Jonathon;
Lera-Ramirez, Manuel;
Cade, Nicholas;
Fourniol, Franck J;
Nedelec, Francois;
Surrey, Thomas;
(2019)
Self-Organization of Minimal Anaphase Spindle Midzone Bundles.
Current Biology
, 29
(13)
pp. 2120-2130.
10.1016/j.cub.2019.05.049.
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Abstract
In anaphase spindles, antiparallel microtubules associate to form tight midzone bundles, as required for functional spindle architecture and correct chromosome segregation. Several proteins selectively bind to these overlaps to control cytokinesis. How midzone bundles assemble is poorly understood. Here, using an in vitro reconstitution approach, we demonstrate that minimal midzone bundles can reliably self-organize in solution from dynamic microtubules, the microtubule crosslinker PRC1, and the motor protein KIF4A. The length of the central antiparallel overlaps in these microtubule bundles is similar to that observed in cells and is controlled by the PRC1/KIF4A ratio. Experiments and computer simulations demonstrate that minimal midzone bundle formation results from promoting antiparallel microtubule crosslinking, stopping microtubule plus-end dynamicity, and motor-driven midzone compaction and alignment. The robustness of this process suggests that a similar self-organization mechanism may contribute to the reorganization of the spindle architecture during the metaphase to anaphase transition in cells.
| Type: | Article |
|---|---|
| Title: | Self-Organization of Minimal Anaphase Spindle Midzone Bundles |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.cub.2019.05.049 |
| Publisher version: | https://doi.org/10.1016/j.cub.2019.05.049 |
| Language: | English |
| Additional information: | This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | Mitotic spindle, anaphase, spindle midzone, microtubule, motor protein, kinesin, self-organization, in vitro reconstitution, computer simulation, Cytosim |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10169764 |
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