O'Connor, L;
Heyderman, R;
(2023)
The challenges of defining the human nasopharyngeal resistome.
Trends in Microbiology
, 31
(8)
pp. 816-831.
10.1016/j.tim.2023.02.008.
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Abstract
The nasopharynx is an important microbial reservoir for the emergence and spread of antibiotic-resistant organisms. The nasopharyngeal resistome is an extensive, adaptable reservoir of antibiotic-resistance genes (ARGs) within this niche. Metagenomic sequencing decodes the genetic material of all organisms within a sample using next-generation technologies, permitting unbiased discovery of novel ARGs and associated mobile genetic elements (MGEs). The challenges of sequencing a low-biomass bacterial sample have limited exploration of the nasopharyngeal resistome. Here, we explore the current understanding of the nasopharyngeal resistome, particularly the role of MGEs in propagating antimicrobial resistance (AMR), explore the advantages and limitations of metagenomic sequencing technologies and bioinformatic pipelines for nasopharyngeal resistome analysis, and highlight the key outstanding questions for future research.
| Type: | Article |
|---|---|
| Title: | The challenges of defining the human nasopharyngeal resistome |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.tim.2023.02.008 |
| Publisher version: | https://doi.org/10.1016/j.tim.2023.02.008 |
| Language: | English |
| Additional information: | © 2023 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| Keywords: | metagenomics, resistome, nasopharyngeal, antimicrobial resistance |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10173832 |
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