Chakraborty, Madhura;
Sivan, Athul;
Biswas, Arikta;
Sinha, Bidisha;
(2022)
Early tension regulation coupled to surface myomerger is necessary for the primary fusion of C2C12 myoblasts.
Frontiers in Physiology
, 13
, Article 976715. 10.3389/fphys.2022.976715.
Preview |
Text
fphys-13-976715.pdf - Published Version Download (5MB) | Preview |
Preview |
Text
DataSheet1_Early tension regulation coupled to surface myomerger is necessary for the primary fusion of C2C12 myoblasts.PDF - Supplemental Material Download (2MB) | Preview |
Abstract
Here, we study the time-dependent regulation of fluctuation–tension during myogenesis and the role of the fusogen, myomerger. We measure nanometric height fluctuations of the basal membrane of C2C12 cells after triggering differentiation. Fusion of cells increases fluctuation–tension but prefers a transient lowering of tension (at ∼2–24 h). Cells fail to fuse if early tension is continuously enhanced by methyl-β-cyclodextrin (MβCD). Perturbing tension regulation also reduces fusion. During this pre-fusion window, cells that finally differentiate usually display lower tension than other non-fusing cells, validating early tension states to be linked to fate decision. Early tension reduction is accompanied by low but gradually increasing level of the surface myomerger. Locally too, regions with higher myomerger intensity display lower tension. However, this negative correlation is lost in the early phase by MβCD-based cholesterol depletion or later as differentiation progresses. We find that with tension and surface-myomerger’s enrichment under these conditions, myomerger clusters become pronouncedly diffused. We, therefore, propose that low tension aided by clustered surface-myomerger at the early phase is crucial for fusion and can be disrupted by cholesterol-reducing molecules, implying the potential to affect muscle health.
| Type: | Article |
|---|---|
| Title: | Early tension regulation coupled to surface myomerger is necessary for the primary fusion of C2C12 myoblasts |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3389/fphys.2022.976715 |
| Publisher version: | https://doi.org/10.3389/fphys.2022.976715 |
| Language: | English |
| Additional information: | opyright © 2022 Chakraborty, Sivan, Biswas and Sinha. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| Keywords: | myoblast fusion, membrane tension, membrane fluctuations, myomerger, cholesterol |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Experimental Epilepsy |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10176626 |
Archive Staff Only
 |
View Item |
