Hill, Daniel;
Choi, Soyoung;
Cordeiro, Maria Francesca;
(2023)
In Vivo Detection of Retinal Ganglion Cell Stress in Rodents with DARC.
Methods in Molecular Biology
, 2708
pp. 123-129.
10.1007/978-1-0716-3409-7_13.
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Abstract
DARC (detection of apoptosing retinal cells) uses fluorescently tagged Annexin A5 to identify retinal apoptosis non-invasively in vivo using a confocal laser scanning ophthalmoscope (cSLO). This can provide insights into the presence and progression of disease pathology and the efficacy of neuroprotective intervention. The methods of administration, imaging, and quantification of DARC, including the operation of the cSLO, are described here.
| Type: | Article |
|---|---|
| Title: | In Vivo Detection of Retinal Ganglion Cell Stress in Rodents with DARC |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-0716-3409-7_13 |
| Publisher version: | https://doi.org/10.1007/978-1-0716-3409-7_13 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Annexin, Annexin A5, Apoptosis, cSLO, DARC, Neurodegeneration, Retina |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10178899 |
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