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Avoiding bias in fluorescence sensor readout

Rusakov, Dmitri A; (2024) Avoiding bias in fluorescence sensor readout. Nature Reviews Neuroscience , 25 pp. 1-2. 10.1038/s41583-023-00768-9. Green open access

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Abstract

Fluorescent indicators can provide quantitative insights into the spatiotemporal dynamics of signalling molecules released by brain circuits. However, a mismatch between the experimental context and the experimental imaging settings often introduces unexpected errors and biases in such measurements. Appreciating this mismatch should help researchers to arrive at unbiased estimates.

Type: Article
Title: Avoiding bias in fluorescence sensor readout
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/s41583-023-00768-9
Publisher version: https://doi.org/10.1038/s41583-023-00768-9
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Experimental Epilepsy
URI: https://discovery-pp.ucl.ac.uk/id/eprint/10181331
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