Capovilla, G;
Castro, KG;
Collani, S;
Kearney, SM;
Kehoe, DM;
Chisholm, SW;
(2023)
Chitin degradation by Synechococcus WH7803.
Scientific Reports
, 13
(1)
, Article 19944. 10.1038/s41598-023-47332-0.
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Abstract
Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures—yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.
| Type: | Article |
|---|---|
| Title: | Chitin degradation by Synechococcus WH7803 |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/s41598-023-47332-0 |
| Publisher version: | https://doi.org/10.1038/s41598-023-47332-0 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third-party material in this article are included in the Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| Keywords: | Ecology, Microbiology, Molecular biology |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10181793 |
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