Li, Yixuan;
Luo, Liuxiong;
Kong, Yingqi;
George, Sophiamma;
Li, Yujia;
Guo, Xiaotong;
Li, Xin;
... Li, Bing; + view all
(2024)
A Point-of-Care Sensing Platform for Multiplexed Detection of Chronic Kidney Disease Biomarkers Using Molecularly Imprinted Polymers.
Advanced Functional Materials
, Article 2316865. 10.1002/adfm.202316865.
(In press).
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Abstract
Chronic kidney disease (CKD) is one of the most serious non-communicable diseases affecting the population. In the early-stages patients have no obvious symptoms, until it becomes life-threatening leading end-stage kidney failure. Therefore, it is important to early diagnose CKD to allow therapeutic interventions and progression monitoring. Here, a point-of-care (POC) sensing platform is reported for the simultaneous detection of three CKD biomarkers, namely creatinine, urea, and human serum albumin (HSA), using reduced graphene oxide/polydopamine-molecularly imprinted polymer (rGO/PDA-MIP) fabricated with novel surface-molecularly imprinting technology. A multi-channel electrochemical POC readout system with differential pulse voltammetry (DPV) function is developed, allowing the simultaneous detection of the three biomarkers, in combination with the surface-MIP electrodes. This sensing platform achieves the record low limit-of-detection (LoD) at a femtomolar level for all three analytes, with wide detection ranges covering their physiological concentrations. Clinical validation is performed by measuring these analytes in serum and urine from healthy controls and patients with CKD. The average recovery rate is 81.8–119.1% compared to the results obtained from the hospital, while this platform is more cost-effective, user-friendly, and requires less sample-to-result time, showing the potential to be deployed in resource-limited settings for the early diagnosis and tracking progression of CKD.
| Type: | Article |
|---|---|
| Title: | A Point-of-Care Sensing Platform for Multiplexed Detection of Chronic Kidney Disease Biomarkers Using Molecularly Imprinted Polymers |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/adfm.202316865 |
| Publisher version: | https://doi.org/10.1002/adfm.202316865 |
| Language: | English |
| Additional information: | Copyright © 2024 The Authors. Advanced Functional Materials published by Wiley-VCH GmbH This is an open access article under the terms of the Creative Commons Attribution License, https://creativecommons.org/licenses/by/4.0/,which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Chronic kidney disease; molecularly imprinted polymer; multiplexed detection; point-of-care biosensor; reduced graphene oxide |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > MAPS Faculty Office UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > MAPS Faculty Office > Institute for Materials Discovery |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10187497 |
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