Michaeli, Lirin; Spector, Eyal; Haeussler, Simon; Carvalho, Cátia A; Grobe, Hanna; Abu-Shach, Ulrike Bening; Zinger, Hen; ... Broday, Limor; + view all Michaeli, Lirin; Spector, Eyal; Haeussler, Simon; Carvalho, Cátia A; Grobe, Hanna; Abu-Shach, Ulrike Bening; Zinger, Hen; Conradt, Barbara; Broday, Limor; - view fewer (2024) ULP-2 SUMO protease regulates UPRmt and mitochondrial homeostasis in Caenorhabditis elegans. Free Radical Biology and Medicine , 214 pp. 19-27. 10.1016/j.freeradbiomed.2024.01.050.

Preview

Text 1-s2.0-S0891584924000583-main.pdf - Published Version Download (7MB) | Preview

Abstract

Mitochondria are the powerhouses of cells, responsible for energy production and regulation of cellular homeostasis. When mitochondrial function is impaired, a stress response termed mitochondrial unfolded protein response (UPRmt) is initiated to restore mitochondrial function. Since mitochondria and UPRmt are implicated in many diseases, it is important to understand UPRmt regulation. In this study, we show that the SUMO protease ULP-2 has a key role in regulating mitochondrial function and UPRmt. Specifically, down-regulation of ulp-2 suppresses UPRmt and reduces mitochondrial membrane potential without significantly affecting cellular ROS. Mitochondrial networks are expanded in ulp-2 null mutants with larger mitochondrial area and increased branching. Moreover, the amount of mitochondrial DNA is increased in ulp-2 mutants. Downregulation of ULP-2 also leads to alterations in expression levels of mitochondrial genes involved in protein import and mtDNA replication, however, mitophagy remains unaltered. In summary, this study demonstrates that ULP-2 is required for mitochondrial homeostasis and the UPRmt.

Download activity - last month

Export as XMLJSONCSV

Download activity - last 12 months

Export as XMLJSONCSV

Downloads by country - last 12 months

Export as CSVXMLJSON

Archive Staff Only

View Item