Costantini, Irene;
Morgan, Leah;
Yang, Jiarui;
Balbastre, Yael;
Varadarajan, Divya;
Pesce, Luca;
Scardigli, Marina;
... Hof, Patrick R; + view all
(2023)
A cellular resolution atlas of Broca's area.
Science Advances
, 9
(41)
, Article eadg3844. 10.1126/sciadv.adg3844.
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Abstract
Brain cells are arranged in laminar, nuclear, or columnar structures, spanning a range of scales. Here, we construct a reliable cell census in the frontal lobe of human cerebral cortex at micrometer resolution in a magnetic resonance imaging (MRI)-referenced system using innovative imaging and analysis methodologies. MRI establishes a macroscopic reference coordinate system of laminar and cytoarchitectural boundaries. Cell counting is obtained with a digital stereological approach on the 3D reconstruction at cellular resolution from a custommade inverted confocal light-sheet fluorescence microscope (LSFM). Mesoscale optical coherence tomography enables the registration of the distorted histological cell typing obtained with LSFM to the MRI-based atlas coordinate system. The outcome is an integrated high-resolution cellular census of Broca's area in a human postmortem specimen, within a whole-brain reference space atlas.
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