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Rnd3 coordinates early steps of cortical neurogenesis through actin-dependent and -independent mechanisms

Pacary, Emilie; Azzarelli, Roberta; Guillemot, Francois; (2013) Rnd3 coordinates early steps of cortical neurogenesis through actin-dependent and -independent mechanisms. Nature Communications , 4 , Article 1635. 10.1038/ncomms2614. Green open access

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Abstract

The generation of neurons by neural stem cells is a highly choreographed process that requires extensive and dynamic remodelling of the cytoskeleton at each step of the process. The atypical RhoGTPase Rnd3 is expressed by progenitors in the embryonic brain but its role in early steps of neurogenesis has not been addressed. Here we show that silencing Rnd3 in the embryonic cerebral cortex interferes with the interkinetic nuclear migration of radial glial stem cells, disrupts their apical attachment and modifies the orientation of their cleavage plane. These defects are rescued by co-expression of a constitutively active form of cofilin, demonstrating that Rnd3-mediated disassembly of actin filaments coordinates the cellular behaviour of radial glial. Rnd3 also limits the divisions of basal progenitors via a distinct mechanism involving the suppression of cyclin D1 translation. Interestingly, although Rnd3 expression is controlled transcriptionally by Ascl1, this proneural factor is itself required in radial glial progenitors only for proper orientation of cell divisions. © 2013 Macmillan Publishers Limited. All rights reserved.

Type: Article
Title: Rnd3 coordinates early steps of cortical neurogenesis through actin-dependent and -independent mechanisms
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/ncomms2614
Publisher version: https://doi.org/10.1038/ncomms2614
Language: English
Additional information: This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
URI: https://discovery-pp.ucl.ac.uk/id/eprint/10189171
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