Cameron, George;
Gruszka, Dominika T;
Gruar, Rhian;
Xie, Sherry;
Kaya, Çağla;
Nasmyth, Kim A;
Baxter, Jonathan;
... Yardimci, Hasan; + view all
(2024)
Sister chromatid cohesion establishment during DNA replication termination.
Science
, 384
(6691)
, Article eadf0224. 10.1126/science.adf0224.
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Abstract
Newly copied sister chromatids are tethered together by the cohesin complex, but how sister chromatid cohesion is coordinated with DNA replication is poorly understood. Prevailing models suggest cohesin complexes, bound to DNA before replication, remain behind the advancing replication fork to keep sister chromatids together. By visualizing single replication forks colliding with pre-loaded cohesin complexes, we find that the replisome instead pushes cohesin to where a converging replisome is met. While the converging replisomes are removed during DNA replication termination, cohesin remains on nascent DNA and provides cohesion. Additionally, we show that CMG disassembly during replication termination is vital for proper cohesion in budding yeast. Together, our results support a new model where sister chromatid cohesion is established during DNA replication termination.
| Type: | Article |
|---|---|
| Title: | Sister chromatid cohesion establishment during DNA replication termination |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1126/science.adf0224 |
| Publisher version: | http://dx.doi.org/10.1126/science.adf0224 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Chromatids, Cell Cycle Proteins, Chromosomal Proteins, Non-Histone, DNA Replication, Cohesins, DNA, Saccharomyces cerevisiae Proteins |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10191043 |
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