Yekeler, Humeyra Betul;
Guler, Ece;
Beato, Patricia Santos;
Priya, Sushma;
Abobakr, Fatima Khaled Mohammed;
Dogan, Murat;
Uner, Burcu;
... Cam, Muhammet Emin; + view all
(2024)
Design and in vitro evaluation of curcumin-loaded PLGA nanoparticle embedded sodium alginate/gelatin 3D printed scaffolds for Alzheimer's disease.
International Journal of Biological Macromolecules
, Article 131841. 10.1016/j.ijbiomac.2024.131841.
(In press).
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IJBM-Revised Manuscript (clean version).pdf - Accepted Version Access restricted to UCL open access staff until 27 April 2025. Download (8MB) |
Abstract
Background: Targeted nanoparticles (NPs) are aimed at improving clinical outcomes by enhancing the diagnostic and therapeutic efficacy of drugs in the treatment of Alzheimer's disease (AD). // Methods: Curcumin (CUR)-loaded poly-lactic-co-glycolic acid (PLGA) NPs (CNPs) were produced to demonstrate a prolonged release and successfully embedded into 3D printed sodium alginate (SA)/gelatin (GEL) scaffolds that can dissolve rapidly sublingually. Characterization and in vitro activity of the NPs and scaffolds were evaluated. // Results: Based on the in vitro drug release studies, 99.6 % of the encapsulated CUR was released in a controlled manner within 18 days for the CNPs. In vitro cell culture studies showed that all samples exhibited cell viability above 84.2 % and no significant cytotoxic effect on SH-SY5Y cells. The samples were analyzed through 2 different pathways by PCR analysis. Real-time PCR results indicated that CNP and CNP-embedded SA/GEL scaffolds (CNPSGS) may show neuroprotective effects by modulating the Wnt/β-catenin pathway. The gene expression level of β-catenin slightly increased compared to the gene expression levels of other proteins and enzymes with these treatments. However, the PI3K/Akt/GSK-3β signaling pathway was regulated at the same time because of the crosstalk between these 2 pathways. // Conclusion: CNPSGS might be an effective therapeutic alternative for AD treatment.
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