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Production, characterization, and in vitro evaluation of anti-Alzheimer's Memantine/Donepezil/Metformin-loaded PVA/PEO composite nanofibers by pressurized gyration

Guler, Ece; Kopuz, Aysen; Ertas, Busra; Yilmaz-Goler, Ayse Mine; Yazir, Yusufhan; Kalaskar, Deepak M; Cam, Muhammet Emin; (2024) Production, characterization, and in vitro evaluation of anti-Alzheimer's Memantine/Donepezil/Metformin-loaded PVA/PEO composite nanofibers by pressurized gyration. Journal of Drug Delivery Science and Technology , 97 , Article 105795. 10.1016/j.jddst.2024.105795.

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Abstract

In this study, memantine (MM) and donepezil (DO) were combined with metformin (MF) as a new approach to Alzheimer's disease (AD) treatment sublingually. MM, DO, and MF were loaded in polyvinyl alcohol (PVA)/polyethylene oxide (PEO) composite nanofibers (NFs), which were produced in three different ratios (9/1, 8/2, and 7/3) by pressurized gyration. The diameter of fibers was decreased by reducing the PEO ratios and adding the drugs in polymer solutions. MM, DO, and MF were successfully loaded and distributed in NFs by the results of FTIR, XRD, and DSC tests. The drug release percentages were enhanced with an increasing ratio of PVA in fibers by the dissolution test results. All fibers at various ratios were found suitable for the sublingual route by disintegrating quickly. No significant toxicity of nanofibers at 24 and 48 h was observed on SH-SY5Y cells. The highest neuroprotection activity was observed in the cells treated with MM/DO/MF-loaded NFs at 7/3. The mRNAs of APP and BACE-1 of the MM/DO/MF-loaded NF group were lower than those of the Aβ1-42 group. While, ADAM-10 mRNA expression increased in the MM/DO/MF-loaded NFs treatment group via PCR results. The morphological structure of the cells treated with the MM/DO/MF-loaded NFs was protected from Aβ1-42-induced neurotoxicity according to confocal laser scanning microscopy images.

Type: Article
Title: Production, characterization, and in vitro evaluation of anti-Alzheimer's Memantine/Donepezil/Metformin-loaded PVA/PEO composite nanofibers by pressurized gyration
DOI: 10.1016/j.jddst.2024.105795
Publisher version: http://dx.doi.org/10.1016/j.jddst.2024.105795
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions.
Keywords: Alzheimer’s disease, Memantine, Donepezil, Metformin, Pressurized gyration, Nanofiber
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Ortho and MSK Science
URI: https://discovery-pp.ucl.ac.uk/id/eprint/10192644
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