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Characterisation of cell membrane interaction mechanisms of antimicrobial peptides by electrical bilayer recording

Priyadarshini, D; Ivica, J; Separovic, F; de Planque, MRR; (2022) Characterisation of cell membrane interaction mechanisms of antimicrobial peptides by electrical bilayer recording. Biophysical Chemistry , 281 , Article 106721. 10.1016/j.bpc.2021.106721. Green open access

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Abstract

Many antimicrobial peptides (AMPs) are cationic host defence peptides (HDPs) that interact with microbial membranes. This ability may lead to implementation of AMPs as therapeutics to overcome the wide-spread antibiotic resistance problem as the affected bacteria may not be able to recover from membrane lysis types of attack. AMP interactions with lipid bilayer membranes are typically explained through three mechanisms, i.e., barrel-stave pore, toroidal pore and carpet models. Electrical bilayer recording is a relatively simple and sensitive technique that is able to capture the nanoscale perturbations caused by the AMPs in the bilayer membranes. Molecular-level understanding of the behaviour of AMPs in relation to lipid bilayers mimicking bacterial and human cell membranes is essential for their development as novel therapeutic agents that are capable of targeted action against disease causing micro-organisms. The effects of four AMPs (aurein 1.2, caerin 1.1, citropin 1.1 and maculatin 1.1 from the skin secretions of Australian tree frogs) and the toxin melittin (found in the venom of honeybees) on two different phospholipid membranes were studied using the electrical bilayer recording technique. Bilayers composed of zwitterionic (DPhPC) and anionic (DPhPC/POPG) lipids were used to mimic the charge of eukaryotic and prokaryotic cell membranes, respectively, so as to determine the corresponding interaction mechanisms for different concentrations of the peptide. Analysis of the dataset corresponding to the four frog AMPs, as well as the resulting dataset corresponding to the bee toxin, confirms the proposed peptide-bilayer interaction models in existing publications and demonstrates the importance of using appropriate bilayer compositions and peptide concentrations for AMP studies.

Type: Article
Title: Characterisation of cell membrane interaction mechanisms of antimicrobial peptides by electrical bilayer recording
Location: Netherlands
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.bpc.2021.106721
Publisher version: http://dx.doi.org/10.1016/j.bpc.2021.106721
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Life Sciences & Biomedicine, Physical Sciences, Biochemistry & Molecular Biology, Biophysics, Chemistry, Physical, Chemistry, Antimicrobial peptides, Bilayer lipid membranes, Lipid-peptide interactions, Phospholipids, AUREIN 1.2, MELITTIN, ENHANCE, MODEL
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: https://discovery-pp.ucl.ac.uk/id/eprint/10194399
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