Meng, Yuxuan;
Li, Changtai;
Patient, Gregory S;
Tian, Yuan;
Chu, Colin J;
(2024)
Imaging Cell Signaling in Tissues Using the IBEX Method.
Methods in Molecular Biology
, 2800
pp. 67-74.
10.1007/978-1-0716-3834-7_6.
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Abstract
The study of cell signaling within tissues can be enhanced using highly multiplexed immunohistochemistry to localize the presence and spatial distribution of numerous pathways of interest simultaneously. Additional data can also be gained by placing the identified proteins into the context of adjacent structures, stroma, and interacting partners. Here, we outline a protocol for using the recently described IBEX method on tissues. This is an open and simple cyclic immunohistochemistry approach suited to this application. We describe a simplified protocol and provide guidance on the method, using a 12-marker panel on human retina to demonstrate the approach.
| Type: | Article |
|---|---|
| Title: | Imaging Cell Signaling in Tissues Using the IBEX Method |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-0716-3834-7_6 |
| Publisher version: | https://doi.org/10.1007/978-1-0716-3834-7_6 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Cell signaling, IBEX, Immunofluorescence, Immunohistochemistry, Retina |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10195315 |
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