Cooksley, Grace;
Dymond, Marcus K;
Stewart, Nicolas A;
Bucca, Giselda;
Hesketh, Andrew;
Lacey, Joseph;
Gogotsi, Yury;
(2023)
Positive resolution of the wound-healing response in lens epithelial cells by Ti3C2Tx MXene coatings for use in accommodative intraocular lens devices.
2D Materials
, 10
(1)
, Article 014003. 10.1088/2053-1583/ac95a7.
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Abstract
Cataract surgery removes the diseased lens of the eye replacing it with an intraocular lens, restoring visual acuity. However, accommodation, the lens' ability to provide dynamic change in focus, is lost. A number of accommodative intraocular lens (AIOL) designs have been considered although none have provided a truly effective clinical AIOL. Two-dimensional titanium carbide (Ti3C2Tx) MXene has been used as a transparent conductive electrode within an AIOL feasibility study. Nevertheless, the potential for Ti3C2Tx to repress excessive inflammation and promote wound healing following cataract surgery has not been considered. Cataract surgery can trigger chronic inflammation and epithelial-mesenchymal transition (EMT) in residual lens epithelial cells (LECs), producing a fibrotic mass across the posterior capsule known as posterior capsule opacification (PCO). With a large surface area and capacity for surface functionalisation, MXene has properties enabling a dual purpose AIOL design with an additional therapeutic role in the repression of pathways leading to PCO development. In this study, Ti3C2Tx MXene was investigated to determine its impact on pathways leading to chronic inflammation and EMT using an in vitro LECs model. Ti3C2Tx MXene was synthesised and characterised using UV-vis spectroscopy, dynamic light scattering and scanning electron microscopy. Changes in markers linked to inflammation and EMT in Ti3C2Tx-treated LECs were measured using enzyme linked immunosorbent assays, quantitative polymerase chain reaction, scratch assay, RNA sequencing for whole-cell gene expression profiling and lipidomics analysis. Ti3C2Tx significantly reduced the expression of pro-inflammatory cytokines by interleukin 1 beta primed LECs and did not advocate EMT, promoting a positive resolution of the wound healing response. This study supports the role of Ti3C2Tx within an AIOL design with the potential to repress key developmental pathways leading to PCO.
| Type: | Article |
|---|---|
| Title: | Positive resolution of the wound-healing response in lens epithelial cells by Ti3C2Tx MXene coatings for use in accommodative intraocular lens devices |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1088/2053-1583/ac95a7 |
| Publisher version: | http://dx.doi.org/10.1088/2053-1583/ac95a7 |
| Language: | English |
| Additional information: | Original content from this work may be used under the terms of the Creative Commons Attribution 4.0 license, https://creativecommons.org/licenses/by/4.0/. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. |
| Keywords: | Titanium carbide, MXene, posterior capsule opacification, intraocular lenses, inflammation, wound-healing response |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Biology and Cancer Dept |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10196873 |
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