UCL Discovery Stage
UCL home » Library Services » Electronic resources » UCL Discovery Stage

Archaeal NusA2 is the ancestor of ribosomal protein eS7 in eukaryotes

Phung, Duy Khanh; Pilotto, Simona; Matelska, Dorota; Blombach, Fabian; Pinotsis, Nikos; Hovan, Ladislav; Gervasio, Francesco Luigi; (2024) Archaeal NusA2 is the ancestor of ribosomal protein eS7 in eukaryotes. Structure 10.1016/j.str.2024.10.019. (In press). Green open access

[thumbnail of Blombach_1-s2.0-S0969212624004544-main.pdf]
Preview
Text
Blombach_1-s2.0-S0969212624004544-main.pdf

Download (3MB) | Preview

Abstract

N-utilization substance A (NusA) is a regulatory factor with pleiotropic functions in gene expression in bacteria. Archaea encode two conserved small proteins, NusA1 and NusA2, with domains orthologous to the two RNA binding K Homology (KH) domains of NusA. Here, we report the crystal structures of NusA2 from Sulfolobus acidocaldarius and Saccharolobus solfataricus obtained at 3.1 Å and 1.68 Å, respectively. NusA2 comprises an N-terminal zinc finger followed by two KH-like domains lacking the GXXG signature. Despite the loss of the GXXG motif, NusA2 binds single-stranded RNA. Mutations in the zinc finger domain compromise the structural integrity of NusA2 at high temperatures and molecular dynamics simulations indicate that zinc binding provides an energy barrier preventing the domain from reaching unfolded states. A structure-guided phylogenetic analysis of the KH-like domains supports the notion that the NusA2 clade is ancestral to the ribosomal protein eS7 in eukaryotes, implying a potential role of NusA2 in translation.

Type: Article
Title: Archaeal NusA2 is the ancestor of ribosomal protein eS7 in eukaryotes
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.str.2024.10.019
Publisher version: https://doi.org/10.1016/j.str.2024.10.019
Language: English
Additional information: Copyright © 2024 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords: Archaea; NusA; RNA-binding protein; transcription; translation; evolution; eS7; S7e
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: https://discovery-pp.ucl.ac.uk/id/eprint/10200088
Downloads since deposit
180Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item