St�hmer, T;
Amar, M;
Harvey, RJ;
Bermudez, I;
Van Minnen, J;
Darlison, MG;
(1996)
Structure and pharmacological properties of a molluscan glutamate-gated cation channel and its likely role in feeding behavior.
Journal of Neuroscience
, 16
(9)
2869 - 2880.
![[thumbnail of STRUCTURE_AND_PHARMACOLOGICAL._PDF.pdf]](https://discovery-pp.ucl.ac.uk/style/images/fileicons/application_pdf.png) Preview |
PDF
STRUCTURE_AND_PHARMACOLOGICAL._PDF.pdf Available under License : See the attached licence file. Download (2MB) |
Abstract
We describe the isolation of a molluscan (Lymnaea stagnalis) full-length complementary DNA that encodes a mature polypeptide (which we have named Lym-eGluR2) with a predicted molecular weight of 105 kDa that exhibits 44-48% identity to the mammalian kainate-selective glutamate receptor GluR5, GluR6, and GluR7 subunits. Injection of in vitro-transcribed RNA from this clone into Xenopus laevis oocytes results in the robust expression of homo-oligomeric cation channels that can be gated by L-glutamate (EC50 = 1.2 +/- 0.3 micron) and several other glutamate receptor agonists; rank order of potency: glutamate >> kainate > ibotenate > AMPA. These currents can be blocked by the mammalian non-NMDA receptor antagonists 6,7-dinitroquinoxaline-2,3-dione, 6-cyano-7-nitroquinoxaline-2,3-dione, and 1-(4-chlorobenzoyl)piperazine-2,3-dicarboxylic acid. Ionic-replacement experiments have shown that the agonist-induced current is carried entirely by sodium and potassium ions. In situ hybridization has revealed that the Lym-eGluR2 transcript is present in all 11 ganglia of the Lymnaea CNS, including the 4-cluster motorneurons within the paired buccal ganglia. The pharmacological properties and deduced location of Lym-eGluR2 are entirely consistent with it being (a component of) the receptor, which has been identified previously on buccal motorneurons, that mediates the excitatory effects of glutamate released from neurons within the feeding central pattern generator.
| Type: | Article |
|---|---|
| Title: | Structure and pharmacological properties of a molluscan glutamate-gated cation channel and its likely role in feeding behavior. |
| Open access status: | An open access version is available from UCL Discovery |
| Publisher version: | http://www.jneurosci.org/content/16/9/2869.full |
| Language: | English |
| Additional information: | This work is available to the public to copy, distribute, or display under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/1351291 |
Archive Staff Only
 |
View Item |
