Massignani, M;
Canton, I;
Sun, T;
Hearnden, V;
Macneil, S;
Blanazs, A;
Armes, SP;
... Battaglia, G; + view all
(2010)
Enhanced fluorescence imaging of live cells by effective cytosolic delivery of probes.
PLoS One
, 5
(5)
, Article e10459. 10.1371/journal.pone.0010459.
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Abstract
Microscopic techniques enable real-space imaging of complex biological events and processes. They have become an essential tool to confirm and complement hypotheses made by biomedical scientists and also allow the re-examination of existing models, hence influencing future investigations. Particularly imaging live cells is crucial for an improved understanding of dynamic biological processes, however hitherto live cell imaging has been limited by the necessity to introduce probes within a cell without altering its physiological and structural integrity. We demonstrate herein that this hurdle can be overcome by effective cytosolic delivery.
| Type: | Article |
|---|---|
| Title: | Enhanced fluorescence imaging of live cells by effective cytosolic delivery of probes. |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1371/journal.pone.0010459 |
| Publisher version: | http://dx.doi.org/10.1371/journal.pone.0010459 |
| Language: | English |
| Additional information: | © 2010 Massignani et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. PMCID: PMC2862714 |
| Keywords: | Cell Death, Cell Survival, Cells, Cultured, Cytosol, Fibrin, Fibroblasts, Fluorescence, Humans, Intracellular Space, Models, Biological, Molecular Imaging, Molecular Probes, Polymers, Rhodamines, Staining and Labeling |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/1387218 |
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