Luft, C;
Freeman, J;
Elliott, D;
Al-Tamimi, N;
Kriston-Vizi, J;
Heintze, J;
Lindenschmidt, I;
... Ketteler, R; + view all
(2014)
Application of Gaussia luciferase in bicistronic and non-conventional secretion reporter constructs.
BMC Biochemistry
, 15
, Article 14. 10.1186/1471-2091-15-14.
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Abstract
Background: Secreted luciferases are highly useful bioluminescent reporters for cell-based assays and drug discovery. A variety of secreted luciferases from marine organisms have been described that harbor an N-terminal signal peptide for release along the classical secretory pathway. Here, we have characterized the secretion of Gaussia luciferase in more detail. / Results: We describe three basic mechanisms by which GLUC can be released from cells: first, classical secretion by virtue of the N-terminal signal peptide; second, internal signal peptide-mediated secretion and third, non-conventional secretion in the absence of an N-terminal signal peptide. Non-conventional release of dNGLUC is not stress-induced, does not require autophagy and can be enhanced by growth factor stimulation. Furthermore, we have identified the golgi-associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1) as a suppressor of release of dNGLUC. / Conclusions: Due to its secretion via multiple secretion pathways GLUC can find multiple applications as a research tool to study classical and non-conventional secretion. As GLUC can also be released from a reporter construct by internal signal peptide-mediated secretion it can be incorporated in a novel bicistronic secretion system.
| Type: | Article |
|---|---|
| Title: | Application of Gaussia luciferase in bicistronic and non-conventional secretion reporter constructs |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1186/1471-2091-15-14 |
| Publisher version: | http://dx.doi.org/10.1186/1471-2091-15-14 |
| Language: | English |
| Additional information: | © Luft et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
| Keywords: | ATP-Binding Cassette Transporters, Adaptor Proteins, Vesicular Transport, Bacterial Proteins, Biochemistry, Bodily Secretions, Genes, Genes, Reporter, HEK293 Cells, Humans, Luciferases, Firefly, Protein Sorting Signals |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/1434641 |
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