Tomlinson, PR;
Zheng, Y;
Fischer, R;
Heidasch, R;
Gardiner, C;
Evetts, S;
Hu, M;
... Morris, J; + view all
(2015)
Identification of distinct circulating exosomes in Parkinson's disease.
Annals of Clinical and Translational Neurology
, 2
(4)
353 - 361.
10.1002/acn3.175.
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Spreadsheet (Supplementary table 3)
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Spreadsheet (Supplementary table 4)
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Abstract
OBJECTIVE: Whether circulating microvesicles convey bioactive signals in neurodegenerative diseases remains currently unknown. In this study, we investigated the biochemical composition and biological function of exosomes isolated from sera of patients with Parkinson's disease (PD). METHODS: Proteomic analysis was performed on microvesicle preparations from grouped samples of patients with genetic and sporadic forms of PD, amyotrophic lateral sclerosis, and healthy subjects. Nanoparticle-tracking analysis was used to assess the number and size of exosomes between patient groups. To interrogate their biological effect, microvesicles were added to primary rat cortical neurons subjected to either nutrient deprivation or sodium arsenite. RESULTS: Among 1033 proteins identified, 23 exosome-associated proteins were differentially abundant in PD, including the regulator of exosome biogenesis syntenin 1. These protein changes were detected despite similar exosome numbers across groups suggesting that they may reflect exosome subpopulations with distinct functions. Accordingly, we showed in models of neuronal stress that Parkinson's-derived microvesicles have a protective effect. INTERPRETATION: Collectively, these data suggest for the first time that immunophenotyping of circulating exosome subpopulations in PD may lead to a better understanding of the systemic response to neurodegeneration and the development of novel therapeutics.
| Type: | Article |
|---|---|
| Title: | Identification of distinct circulating exosomes in Parkinson's disease |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/acn3.175 |
| Publisher version: | http://dx.doi.org/10.1002/acn3.175 |
| Language: | English |
| Additional information: | Copyright © 2015 The Authors. Annals of Clinical and Translational Neurology published by Wiley Periodicals, Inc on behalf of American Neurological Association. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/1468493 |
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