Baud, D;
Ladkau, N;
Moody, TS;
Ward, JM;
Hailes, HC;
(2015)
A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase.
Chemical Communications
, 51
(97)
pp. 17225-17228.
10.1039/c5cc06817g.
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Abstract
A new colorimetric method has been developed to screen transaminases using an inexpensive amine donor. The assay is sensitive, has a low level of background coloration, and can be used to identify and profile transaminase activities against aldehyde and ketone substrates in a high-throughput format. Significantly it is also amendable to solid phase colony screening.
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