Caxaria, SMGL; (2016) Induced pluripotent stem cells (iPSCs) for research and therapy: induction of hepatic differentiation in iPSCs and evaluation of their quality as a model of in vivo development in the context of coagulation. Doctoral thesis , UCL (University College London).

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Abstract

Stem cells hold great promise for regenerative medicine as they have the potential to repair almost any tissue. The use of induced pluripotent stem cells (iPSC) offers several advantages over human embryonic stem cells (hESC). Nevertheless, an issue that has slowed the use of iPSC (as well as hESC) in the clinic is safety. The pluripotent capacity that gives these cells their regenerative potential also gives them tumorigenic abilities. Moreover, the reprogramming procedure of iPSC can also affect the quality and safety of the final population. In the search for safer iPSC, we optimized an integration free method of reprogramming that is GMP compliant, which represents a step closer to their clinical use. Hepatocyte-like cells derived from iPSC have proven useful in research, as in disease modelling and toxicity screening, and in the context of cellular therapy could represent a breakthrough for the treatment of liver disorders. We worked on an optimized method that allows quick and efficient hepatocyte differentiation from iPSC. We used this as a model to tap into embryonic development in the context of coagulation, and into the regulators of coagulation, with the goal to better understand coagulation. Similar studies can then be used to study other pathways besides coagulation and help increase the knowledge on the liver and its many functions.

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