Mokhtar, H;
Pedrera, M;
Frossard, J-P;
Biffar, L;
Hammer, SE;
Kvisgaard, LK;
Larsen, LE;
... Graham, SP; + view all
(2016)
The Non-structural Protein 5 and Matrix Protein Are Antigenic Targets of T Cell Immunity to Genotype 1 Porcine Reproductive and Respiratory Syndrome Viruses.
Frontiers In Immunology
, 7
, Article 40. 10.3389/fimmu.2016.00040.
Preview |
Text
Mokhtar_Non-structural Protein 5 and Matrix Protein Are Antigenic Targets of T Cell Immunity.pdf Download (3MB) | Preview |
Abstract
The porcine reproductive and respiratory syndrome virus (PRRSV) is the cause of one of the most economically important diseases affecting swine worldwide. Efforts to develop a next-generation vaccine have largely focused on envelope glycoproteins to target virus-neutralizing antibody responses. However, these approaches have failed to demonstrate the necessary efficacy to progress toward market. T cells are crucial to the control of many viruses through cytolysis and cytokine secretion. Since control of PRRSV infection is not dependent on the development of neutralizing antibodies, it has been proposed that T cell-mediated immunity plays a key role. Therefore, we hypothesized that conserved T cell antigens represent prime candidates for the development a novel PRRS vaccine. Antigens were identified by screening a proteome-wide synthetic peptide library with T cells from cohorts of pigs rendered immune by experimental infections with a closely related (subtype 1) or divergent (subtype 3) PRRSV-1 strain. Dominant T cell IFN-γ responses were directed against the non-structural protein 5 (NSP5), and to a lesser extent, the matrix (M) protein. The majority of NSP5-specific CD8 T cells and M-specific CD4 T cells expressed a putative effector memory phenotype and were polyfunctional as assessed by coexpression of TNF-α and mobilization of the cytotoxic degranulation marker CD107a. Both antigens were generally well conserved among strains of both PRRSV genotypes. Thus, M and NSP5 represent attractive vaccine candidate T cell antigens, which should be evaluated further in the context of PRRSV vaccine development.
| Type: | Article |
|---|---|
| Title: | The Non-structural Protein 5 and Matrix Protein Are Antigenic Targets of T Cell Immunity to Genotype 1 Porcine Reproductive and Respiratory Syndrome Viruses |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3389/fimmu.2016.00040 |
| Publisher version: | http://dx.doi.org/10.3389/fimmu.2016.00040 |
| Language: | English |
| Additional information: | Copyright © 2016 Mokhtar, Pedrera, Frossard, Biffar, Hammer, Kvisgaard, Larsen, Stewart, Somavarapu, Steinbach and Graham. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Immunology, porcine reproductive and respiratory syndrome virus, T cell, IFN-gamma, antigen identification, phenotype and function, vaccine, Experimental Challenge, Lymphocyte Subsets, Clinical-disease, Syndrome Prrs, Ifn-gamma, Pigs, Vaccine, Responses, Swine, Identification |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/1478873 |
Archive Staff Only
 |
View Item |
