Heywood, WE;
Pryce, J;
Virasami, A;
Preece, RL;
Dezateux, C;
Mills, K;
Sebire, NJ;
(2016)
A rapid high throughput proteomic method based on profiling of proteolytic free peptides to assess post-delivery degradation of placental tissue.
Placenta
, 44
pp. 109-111.
10.1016/j.placenta.2016.04.014.
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Heywood et al A rapid high throughput proteomic method based on profiling of proteolytic free peptides to assess post-delivery degradation of placental tissue VoR.pdf Download (507kB) | Preview |
Abstract
A rapid method to determine quality for placental proteomic studies is required due to varying lengths of time between delivery and sampling in routine protocols. We developed a rapid 10 min LC-MS based scanning method to profile free peptides liberated from natural proteolytic degradation. The assay was applied to placenta samples obtained following refrigeration for varying time periods post-delivery (12 h, +24 h, +48 h and +72 h). Analysis reveals time dependant overlapping profiles for groups <24 to +48 h with greatest variation in the +72 h group, indicating that significant proteolysis affects tissue integrity between 48 and 72 h.
| Type: | Article |
|---|---|
| Title: | A rapid high throughput proteomic method based on profiling of proteolytic free peptides to assess post-delivery degradation of placental tissue |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.placenta.2016.04.014 |
| Publisher version: | http://dx.doi.org/10.1016/j.placenta.2016.04.014 |
| Language: | English |
| Additional information: | Copyright © 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International licence (CC BY-NC-ND 4.0) (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| Keywords: | Free peptide, LC-MS, Multivariate analysis, Placenta, Proteolysis |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Genetics and Genomic Medicine Dept UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Population, Policy and Practice Dept |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/1494669 |
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