Miyazaki, Y;
Mizumoto, K;
Dey, G;
Kudo, T;
Perrino, J;
Chen, L-C;
Meyer, T;
(2016)
A method to rapidly create protein aggregates in living cells.
Nature Communications
, 7
, Article 11689. 10.1038/ncomms11689.
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Abstract
The accumulation of protein aggregates is a common pathological hallmark of many neurodegenerative diseases. However, we do not fully understand how aggregates are formed or the complex network of chaperones, proteasomes and other regulatory factors involved in their clearance. Here, we report a chemically controllable fluorescent protein that enables us to rapidly produce small aggregates inside living cells on the order of seconds, as well as monitor the movement and coalescence of individual aggregates into larger structures. This method can be applied to diverse experimental systems, including live animals, and may prove valuable for understanding cellular responses and diseases associated with protein aggregates.
| Type: | Article |
|---|---|
| Title: | A method to rapidly create protein aggregates in living cells |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/ncomms11689 |
| Publisher version: | http://dx.doi.org/10.1038/ncomms11689 |
| Language: | English |
| Additional information: | Copyright © The Author(s). All rights reserved. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| Keywords: | Science & Technology, Multidisciplinary Sciences, Misfolded Proteins, Caenorhabditis-Elegans, Small Molecules, Proteasome, Trafficking, Stability, Nuclear, Stress |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/1500953 |
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