Kriston-Vizi, J;
Flotow, H;
(2016)
Getting the whole picture: High content screening using three-dimensional cellular model systems and whole animal assays.
Cytometry Part A
, 91
p. 159.
10.1002/cyto.a.22907.
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Abstract
Phenotypic or High Content Screening (HCS) is becoming more widely used for primary screening campaigns in drug discovery. Currently the vast majority of HCS campaigns are using cell lines grown in well-established monolayer cultures (2D tissue culture). There is widespread recognition that the more biologically relevant 3D tissue culture technologies such as spheroids and organoids and even whole animal assays will eventually be run as primary HCS. Upgrading the IT infrastructure to cope with the increase in data volumes requires investments in hardware (and software) and this will be manageable. However, the main bottleneck for the effective adoption and use of 3D tissue culture and whole animal assays in HCS is anticipated to be the development of software for the analysis of 3D images. In this review we summarize the current state of the available software and how they may be applied to analyzing 3D images obtained from a HCS campaign.
| Type: | Article |
|---|---|
| Title: | Getting the whole picture: High content screening using three-dimensional cellular model systems and whole animal assays |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/cyto.a.22907 |
| Publisher version: | http://dx.doi.org/10.1002/cyto.a.22907 |
| Language: | English |
| Additional information: | This is the peer reviewed version of the following article: Kriston-Vizi, J; Flotow, H; (2016) Getting the whole picture: High content screening using three-dimensional cellular model systems and whole animal assays. Cytometry Part A, which has been published in final form at: http://dx.doi.org/10.1002/cyto.a.22907. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html#terms). |
| Keywords: | 3D tissue culture, automated image analysis, high content screening, phenotypic assays |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/1504669 |
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